A&P lab midterm
Content
A& P Lab notes for exam
Lab # 1
1. Blood functions.
a. Blood functions as transportation, regulation and protection.
2. Blood types and Rh determination.
a. Type A- has A-antigen and genotypes IAIA or IA i. Compatible donors are A
or O. It also has anti-B antibodies. Incompatible with B and AB type.
b. Type B- has B-antigen and genotypes IBIB or IB i. Compatible donors are B
or O. It also has anti-A antibodies. Incompatible with A and AB type.
c. Type AB (universal recipient)- has A and B-antigens and genotype IAIB.
Compatible donors are A, B, AB and O. none antibodies.
d. Type O (universal donor)- has none antigens and genotype is ii. Has A and
B antibodies. Compatible donors are type O. Incompatible donors are A,
B, AB.
e. Rh is determined using the anti-D serum. This gives the + or – signs. If the
blood coagulates when mixed then its Rh+ if not then is Rh-. If anti-D
agglutinates with blood then it is Rh+.
3. Danger of unmatched transfusion.
a. Mismatched transfusion causes a transfusion reaction and that is the
agglutinated RBCs block small blood vessels, hemolyze, and release their
hemoglobin over the next few hours to days. Free hemoglobin can block
the kidney tubules and cause death from acute renal failure within a week
or so.
4. What is the Tallquist test and what it measures?
a. The tallquist test is done with a tallquist paper that has been saturated with
a sample of blood with a tallquist color chart. The tallquist measures the
concentration of hemoglobin in the blood. Males are usually at 12-18 g/ml
and females 12-16 g/ml.
5. What is hematocrit and how is it measured?
a. Hematocrit is the percentage of erythrocytes found in a set volume of
blood. Average for males is 45% and female is 42%. To measure one
places a few drops of blood onto a slide, then take a capillary tube fill it
with blood and seal the same end, place the tube in a microhematocrit
centrifuge for two minutes, then finally take the tube and read it on the
microhematocrit reader.
6. Know why there might be low or high of hematocrit/hemoglobin levels and
why different in male and female.
a. Four reasons why the values tend to be lower in women than in men:
i. Androgens stimulate RBC production, and men have higher
androgen levels than women.
ii. Women of reproductive age have periodic menstrual losses
iii. The hematocrit is inversely proportional to percent body fat, which
is higher in women than in men.
iv. Also in blood clots faster and the skin have fewer blood vessels
than in women.
Lab # 2 Heart
1. Study the sheep parts in lab handout.
2. Study the left and right side of the heart in handout.
3. Know the path that blood follows.
a. Blood comes in through the superior vena cava and inferior vena cava and
enters the right atrium. The right atrium gets filled up with blood, then by
pressure the AV (tricuspid) valves open up allowing blood to flow into the
right ventricle. Once the right ventricle is filled up a signal will sent by the
SA node to contract the muscles of the heart to send the blood upward
through the pulmonary valve (semilunar) up to the pulmonary trunk
through the pulmonary veins into the lungs where it can get oxygenated
and unload CO2. The same thing is happening on the left side except for
instead of tricuspid valves the left side has AV (bicuspid) valves and aortic
(semilunar) valve which then goes to the Aorta and from there distributed
to body. The blood goes to the body instead of the lungs to supply oxygen.
The blood returns from the lungs via pulmonary veins and from the body
via vena cava.
4. What causes the different heart sound?
a. The first two sounds are caused by the cardiac cycle. The first sound (S1lubb) is when the AV valves close and the second (S2-dub) when they
open.
5. What is auscultation and heart murmurs?
a. Auscultation is listening to sound made by the body. A heart murmur is
when there is an irregular heart rhythm due to the blood having a swishing
or whishing sound during a heart bit.
6. Blood pressure concept and how to measure.
a. Fluid flows down their pressure gradient. Study handout.
7. Know normal blood pressure values and hypertension values.
a. Normal blood pressure values are 120 (systole)/75 (diastole).
Hypertension is 140/90.
8. What is the sound of korotkoff?
a. The contraction and relaxation of ventricles as blood is ejected from the
ventricles.
9. Places where pulse is measured and what it is.
a. Temporal- above and lateral to the eyes.
b. Common carotid- on the side of the neck.
c. Brachial- on the medial side of the biceps brachii
d. Femoral- near the ilium.
10. Immediate and long term changes in the cardiovascular system that result
from exercise.
a. Immediate changes
i. Stroke volume increases allowing more blood flow
ii. Faster heart rate
iii. Cardiac output changes
iv. Makes the heart work harder
b. Long term changes
i. Hypertrophy of ventricles
ii. Allows the heart to beat slowly and still maintaining a normal
resting cardiac output.
iii. Increase of stroke volume.
Lab # 1
1. Blood functions.
a. Blood functions as transportation, regulation and protection.
2. Blood types and Rh determination.
a. Type A- has A-antigen and genotypes IAIA or IA i. Compatible donors are A
or O. It also has anti-B antibodies. Incompatible with B and AB type.
b. Type B- has B-antigen and genotypes IBIB or IB i. Compatible donors are B
or O. It also has anti-A antibodies. Incompatible with A and AB type.
c. Type AB (universal recipient)- has A and B-antigens and genotype IAIB.
Compatible donors are A, B, AB and O. none antibodies.
d. Type O (universal donor)- has none antigens and genotype is ii. Has A and
B antibodies. Compatible donors are type O. Incompatible donors are A,
B, AB.
e. Rh is determined using the anti-D serum. This gives the + or – signs. If the
blood coagulates when mixed then its Rh+ if not then is Rh-. If anti-D
agglutinates with blood then it is Rh+.
3. Danger of unmatched transfusion.
a. Mismatched transfusion causes a transfusion reaction and that is the
agglutinated RBCs block small blood vessels, hemolyze, and release their
hemoglobin over the next few hours to days. Free hemoglobin can block
the kidney tubules and cause death from acute renal failure within a week
or so.
4. What is the Tallquist test and what it measures?
a. The tallquist test is done with a tallquist paper that has been saturated with
a sample of blood with a tallquist color chart. The tallquist measures the
concentration of hemoglobin in the blood. Males are usually at 12-18 g/ml
and females 12-16 g/ml.
5. What is hematocrit and how is it measured?
a. Hematocrit is the percentage of erythrocytes found in a set volume of
blood. Average for males is 45% and female is 42%. To measure one
places a few drops of blood onto a slide, then take a capillary tube fill it
with blood and seal the same end, place the tube in a microhematocrit
centrifuge for two minutes, then finally take the tube and read it on the
microhematocrit reader.
6. Know why there might be low or high of hematocrit/hemoglobin levels and
why different in male and female.
a. Four reasons why the values tend to be lower in women than in men:
i. Androgens stimulate RBC production, and men have higher
androgen levels than women.
ii. Women of reproductive age have periodic menstrual losses
iii. The hematocrit is inversely proportional to percent body fat, which
is higher in women than in men.
iv. Also in blood clots faster and the skin have fewer blood vessels
than in women.
Lab # 2 Heart
1. Study the sheep parts in lab handout.
2. Study the left and right side of the heart in handout.
3. Know the path that blood follows.
a. Blood comes in through the superior vena cava and inferior vena cava and
enters the right atrium. The right atrium gets filled up with blood, then by
pressure the AV (tricuspid) valves open up allowing blood to flow into the
right ventricle. Once the right ventricle is filled up a signal will sent by the
SA node to contract the muscles of the heart to send the blood upward
through the pulmonary valve (semilunar) up to the pulmonary trunk
through the pulmonary veins into the lungs where it can get oxygenated
and unload CO2. The same thing is happening on the left side except for
instead of tricuspid valves the left side has AV (bicuspid) valves and aortic
(semilunar) valve which then goes to the Aorta and from there distributed
to body. The blood goes to the body instead of the lungs to supply oxygen.
The blood returns from the lungs via pulmonary veins and from the body
via vena cava.
4. What causes the different heart sound?
a. The first two sounds are caused by the cardiac cycle. The first sound (S1lubb) is when the AV valves close and the second (S2-dub) when they
open.
5. What is auscultation and heart murmurs?
a. Auscultation is listening to sound made by the body. A heart murmur is
when there is an irregular heart rhythm due to the blood having a swishing
or whishing sound during a heart bit.
6. Blood pressure concept and how to measure.
a. Fluid flows down their pressure gradient. Study handout.
7. Know normal blood pressure values and hypertension values.
a. Normal blood pressure values are 120 (systole)/75 (diastole).
Hypertension is 140/90.
8. What is the sound of korotkoff?
a. The contraction and relaxation of ventricles as blood is ejected from the
ventricles.
9. Places where pulse is measured and what it is.
a. Temporal- above and lateral to the eyes.
b. Common carotid- on the side of the neck.
c. Brachial- on the medial side of the biceps brachii
d. Femoral- near the ilium.
10. Immediate and long term changes in the cardiovascular system that result
from exercise.
a. Immediate changes
i. Stroke volume increases allowing more blood flow
ii. Faster heart rate
iii. Cardiac output changes
iv. Makes the heart work harder
b. Long term changes
i. Hypertrophy of ventricles
ii. Allows the heart to beat slowly and still maintaining a normal
resting cardiac output.
iii. Increase of stroke volume.
