New Products 2012V2

 

New

products

 VOLU  VO LUME ME 2 I 2012

ANTIBODIES, ASSAYS, SMALL MOLECULES, AND INHIBITORS Neuroscience

Cancer

Signaling

Cell Structure

Stem Cell Research

EMD Millipore—with the expertise of Calbiochem ® , Chemicon ®, and Upstate ®

Cancer: Autophagy’s Role

Autophagy is a highly regulated, degradative process that mediates recycling of intracellular components to maintain Autophagy homeostasis or to promote survival under stressful conditions. conditions. The role of autophagy in cancer is multifaceted. On one hand, autophagy promotes promotes tumor cell survival in hypoxic environmen e nvironments ts or in response to cytotoxic therapies. On the other hand, deficits in autophagy in normal tissues can promote cancer initiation by genoto genotoxic xic stress caused by the accumulation of damaged cellular components and pro-inflammatory necrotic cells. Use EMD Millipore’s antibodies, small molecules, inhibitors and cell-based assays for elucidating autophagy mechanisms and identifying activators and inhibitors for this pathway of emerging importance. FEATURED ASSAY FAMI LY:

LentiBrite™ Pre-Packaged Lentiviral Biosensors Lentiviral vector systems are a popular research tool used to introduce gene products into cells, providing advantages over non-viral methods.  Visualize localization localization of LC3, a key component component of autophagosome autophagosome maturation. Human mesenchymal stem cells transduced with RFP-LC3 Biosensor (Catalogue No. 17-10143). The RFP-LC3 displays a cytosolic distribution in fed cells (A), and a punctate distribution in starved autophagic cells (B).

 Visualize proteins proteins within live cells and in vitro  using  using EMD Millipore’s LentiBrite™ Lentiviral Biosensors: Pre-packaged, GFP- & RFP-tagged protein constructs Long-term, stable fluorescent expression Higher efficiency transfection vs. traditional chemicalbased methods Ability to transfect dividing, non-dividing, and difficultto-transfect to-trans fect cell types Non-disruptive towards cellular function with low immunogenicity • • •

•

•

Improper localization of LC3 control mutant. HT-1080 cells were transduced with GFP-LC3-G120A control mutant lentiviral particles (Catalogue No. 17-10189). GFP-LC3 displays a dif fuse distribution in both fed cells (A) and starved autophagic cells (B) with no translocation to a punctate cytoplasmic distribution as characteristic of wild-type LC3.

Watch a time-lapse video of live cells transfected with LentiBrite™ Lentiviral Biosensors! www.millipore.com/autophagyvideo

EMD Millipore is a division of Merck KGaA, Darmstadt, Germany

Epigenetics & Gene Regulation

 

 VOLUME  VOL UME 2 I 2012

FEATURED ASSAY PRODUCTS EMD Millipore’s new LentiBrite™ Lentiviral Biosensors is a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy autophagy,, apoptosis, and cell structure, enables visualization vi sualization under different cell/disease states in live cell and in vitro  analysis.  analysis.

LentiBrite™ Pre-Packaged Lentiviral Biosensors Description

Catalogue No.

LentiBrite™ GFP-LC3 Lentiviral Biosensor

17-10193

LentiBrite™ RFP-LC3 Lentiviral Biosensor

17-10143

LentiBrite™ RFP-LC3 Control Mutant Lentiviral Biosensor

17-10188

LentiBrite™ GFP-LC3 Control Mutant Lentiviral Biosensor

17-10189

LentiBrite™ GFP-Histone H2B Lentiviral Biosensor

17-10229

LentiBrite™ RFP-Histone H2B Lentiviral Biosensor

17-10228

LentiBrite™ GFP-T GFP-Tubulin ubulin Lentiviral Biosensor

17-10206

LentiBrite™ RFP-Tubulin Lentiviral Biosensor

17-10205

LentiBrite™ GFP-β-actin Lentiviral Biosensor

17-10204

LentiBrite™ RFP-β-actin Lentiviral Biosensor

17-10203

LentiBrite™ α-actinin-GFP Lentiviral Biosensor

17-10156

LentiBrite™ α-actinin-RFP Lentiviral Biosensor

17-10196

LentiBrite™ Paxillin-GFP Lentiviral Biosensor

17-10154

LentiBrite™ Paxillin-RFP Lentiviral Biosensor

17-10155

LentiBrite™ GFP-Viment GFP-Vimentin in Lentiviral Biosensor

17-10152

LentiBrite™ RFP-Viment RFP-Vimentin in Lentiviral Biosensor

17-10153

LentiBrite™ Calreticulin-RFP-KDEL Lentiviral Biosensor

17-10146

LentiBrite™ GFP-HMGB GFP-HMGB1 1 Lentiviral Biosensor

17-10147

NEW PRODUCT HIGHLIGHTS ROCK Activity Assay  Catalogue No. CSA001

 Vascular Permeability Assays Catalogue Nos. ECM642, ECM644

Rho Kinase, a serine-threonine kinase, plays an important

Tumor endothelial endothelial cells often proliferate faster than non-

role in calcium sensitization and control of vascular tone.

tumor endothelial cells, leading to differences between

Use our Rho-associated Protein Kinase (ROCK) Activity

tumor vasculature and normal vasculature in morphology,

Assay Kit to quantify purified ROCK activity or to screen

leakiness, and structural abnormalities. These in vitro

potential inhibitors or activators of ROCK or DMPK family

 Vascular Permeability Assays are an efficient efficient system for

kinases. The assay can also be used to assess the potency of pharmacological agents on these kinases.

characterizing vasculature as well as assessing the effects characterizing of chemicals and drug compounds on endothelial cell monolayer integrity, absorption, transport and permeability. The kits are intended for research use only.

120 100

   n    o    i    t    i    b    i    h    n    I    f    o      %

80 ATP 0.1 µM, IC50=0.15 µM ATP 0.5 µM, IC50=0.21 µM ATP 1 µM, IC 50=0.36 µM ATP 10 µM, IC 50=3.7 µM ATP 100 µM, IC50=21 µM

60 40 20 0 -2

-1

0 1 Y27632 Log (µM)

2

3

Determination of IC50 values for ROCK specific inhibitor Y-27632 (Catalogue No. 688000) with ROCK-II: Reactions containing 10 mU ROCK-II and ATP concentrations from 1X to 1000X Km, were incubated for 30 minutes at 30 °C. Data obtained was analyzed by non-linear curve fit with sigmoidal dose response with variable slope.

2

    )    0    0    0    1   x     (

   t    n   u    o    C    t    n    e    c    s    e    r    o   u    l    F

13 12 11 10 9 8 7 6 5 4 3 2 1 0 No Monolayer

No Treatment

100 ng/mL TNF-α

Example staining and permeability analysis of HUVEC monolayers: The “No Monolayer” sample demonstrated high permeability in the absence of an occlusive endothelial cell monolayer. The “No Treatment” sample displayed low FITC-dextran permeability. Following TNF-α treatment, fluorescent count was significantly increased compared to the “No Treatment” sample.

 

 VOLUME  VOL UME 2 I 2012

NEW PRODUCT HIGHLIGHTS Gelatin Degradation Invadopodia Assays Catalogue Nos. ECM670, ECM671

Cell Comb™ for Scratch Assay Catalogue No. 17-10191

The new QCM™ Gelatin Invadopodia Assays provide a

The Cell Comb™ is an easy-to-use, disposable disposable tool

simplified and standardized protocol for affixing a thin

for creating a high density field of scratches on a cell

layer of pre-labeled fluorescein (green) or Cy3 (red) gelatin

monolayer to maximize the area of wound edges, while

to a glass substrate, as well as reagents for co-localizing

leaving sufficient numbers of undamaged cells to migrate

the actin cytoskeleton and nuclei with degradation sites.

into the gap. This form of high density density wounding creates a

This degradation can be quantified by image analysis methods and used to track proteolytic time course studies,

high proportion of migrating cells to quiescent monolayer cells, which permits sensitive, specific detection of

as well as modulator effects on invadopodia i nvadopodia formation and

biochemical events occurring in migrating cells and sheds

ECM degradation.

light on key pathwa pathways ys activated in tumor invasion and

•

Enables time-course visualization and quantificat quantification ion of

metastasis.

matrix degradation by normal and cancerous cell types •

Includes fluorescently-labeled gelatin and reagents for co-localizing the actin cytoskeleton and nuclei with

Cell Comb™ for Scratch Assay: The kit includes 6 combs and 6 rectangular plates for scratch assay.

degradation sites Cell migration across a fluorescently-conjugated matrix. Dark spots are evidence of invadopodia formation and degradation of the fluorescentlyconjugated gelatin.

NEW PRODUCTS Cancer Description

Catalogue No.

PUBLICATION HIGHLIGHT ON NEW ANTIBODIES:

Antibodies Anti-Angiotensinogen, clone EPR2931, Rabbit Monoclonal Anti An ti-A -Ant nter erio iorr gra gradi dien entt pr prote tein in 2 hom homol olog og,, clo clone ne 7A 7A10 10 Anti-Apoptosis-inducing factor 2 (AIFM2) Anti-Atg4A Anti-Atg4A, cl clone EP EPR4122, Ra Rabbit Mo Monoclonal

MABC123 MAB ABC4 C488 ABC69 ABC29 MABC136

Anti-Atg4B

ABC32

Anti-Atg4C

ABC21

Anti-Atg4D

ABC22

Anti-Atg5 Anti-Atg5, clone EPR1755(2), Rabbit Monoclonal Anti-Atg9 Anti-Atg12, clone EPR4800, Ra Rabbit Monoclonal Anti-Atg16L1

ABC14 MABC137 ABC23 MABC135 ABC25

Anti-Atg18 (WIPI-2), clone 2A2

MABC91

Anti-BCAP31, clone 3D11

MABC41

Anti-BIRC6

ABC56

Anti-CASP8-associated protein 2

ABC54

Anti An ti-C -Cas aspa pase se-8 -8 (a (acti ctive ve fo form rm p1 p188 sub subun unit) it),, clo clone ne 2B1 2B12. 2.11 Anti-Cyclin A2, clone 17B8.2 Anti-Cystatin-C

MAB1 MA B107 0754 54 MABC205 ABC20

Anti-DRAM1

MABC62

Anti-DRAM2

MABC64

For published studies using this new product, visit: www.millipore.com

EMD Millipore’s newly released Anti-Anterior gradient protein 2 homolog (AGP2R), clone 7A10 monoclonal antibody has been published in a breast cancer biomarker study. In the D.L. Barraclough et al. paper entitled, The Metastasis-Associated Metastasis-Ass ociated Anterior Gradient 2 Protein Is Correlated with Poor Survival of Breast Cancer Patients** Patients UK cancer researchers, used the clone 7A10 antibody to correlate AGP2R protein expression in breast cancer tumor samples to prognosis. Until recently, recently, AGR2 had been independently reported to be associated with either reduced or increased survival of different groups of patients. This more extensive study with lengthy lengthy patient follow-ups reveals that the presence of AGR2 in the primary tumor was one of o f the most significant independent prognostic indicators in the study and is therefore a possible prognostic indicator of poor patient outcome in breast cancer. *Am. J. Pathol. (2009) 175:1848-1857.

3

 

 VOLUME  VOL UME 2 I 2012

NEW PRODUCTS Cancer Description

Catalogue No.

Catalogue No.

ABC30

Proteins

Anti-FK506-binding protein-like (FKBPL)

ABC88

β-catenin, activated

23-027

Anti-GABA(A) re receptor-associated pr protein-like 1

ABC58

c-Cbl, active

23-041

Anti-Galectin-3

AB10541

Cyclin E1 complex

23-031

Anti-GATE-16

ABC24

HIF-1α

23-045

Anti-HAX-1

ABT65

IkBa, activated

23-028

Kit MDM2 (c-Myc tagged), active

23-043 23-033

Anti-HtrA1, clone ME-1 Anti-HUNK

MABC77 ABC91

Anti-ICAD (DFF) (cleaved form)

ABC27

MDM2/CK1δ, active

23-032

Anti-IDH2

ABC57

p27 complex, activated

23-024

p53

23-034

Anti-Ipaf (NLRC4)

06-1125

Anti-Kallistatin

ABC51

SCFFbw7 complex, active

23-030

Anti-Mitochondrial fission 1 protein (Fis1)

ABC67

SCFSkp2 complex, active

23-023

Anti-Mitofusin-2 (Mfn2)

ABC42

SCFβTrCP1 complex, active

23-026

Anti-Netrin receptor UNC5B

ABC89

Src

23-042

Anti-Noxa

ABC50

UbcH3, active

23-022

Anti-PARP1 (cleaved form)

ABC26

UbcH4, active

23-025

Anti-phospho-Rb (Ser807/Ser811)

ABC132

UbcH5a, active

23-029

Anti-phospho-ULK1 (Ser555)

ABC124

UbcH5c, active

23-035

UBE1, active

23-021

 VHL complex, complex, active active

23-044

Anti-Plk3 Anti-Podoplanin, clone NZ1.2

ABS89 MABC117

Anti-RalA lA--bind ndiing pr protein 1 (R (RalB lBPP1), cl clone 17 17C11.1

MABC18

Kits & Assays

Anti-RIP3, clone 6E6.2

MABC28

FlowCellect™ Autophagy Reagent Pack

Anti-TDAG51 Anti-TIGAR Anti-Traf2 Anti-TRAIL, clone N2B2 Anti-UVRAG

ABC49 AB10545 ABC47 MABC12 AB2960

FlowCe Flo wCelle llect™ ct™ GFP GFP-L -LC3 C3 Re Repor porte terr Aut Autop ophag hagyy Ass Assay ay Kit (CH (CHO) O) PARP1 Enzyme Activity Assay QCM™ Gelatin Invadopodia Assay (Green) QCM™ Gelatin Invadopodia Assay (Red)

CF200097 FCCH100170 FCCH10 0170 17-10149 ECM670 ECM671

LentiBrite ™ GFP-HMGB1 Le Lentiviral Biosensor

17-10147

Lent Le ntiB iBri rite te ™ GFP GFP-L -LC3 C3 Co Cont ntro roll Mut Mutan antt Len Lenti tivi vira rall Bio Biose sens nsor or

17-1 17 -101 0189 89

LentiBrite ™ GFP-LC3 Lentiviral Biosensor

17-10193

Lent Le ntiB iBri rite te ™ RFP RFP-L -LC3 C3 Co Cont ntro roll Mut Mutan antt Len Lenti tivi vira rall Bio Biose sens nsor or

17-1 17 -101 0188 88

LentiBrite ™ RFP-LC3 Lentiviral Biosensor

17-10143

Lent Le ntiB iBri rite te™ ™ Cal Calre reti ticu culi linn-RF RFPP-K -KDE DELL Len Lenti tivi vira rall Bio Biose sens nsor or

17-1 17 -101 0146 46

PUBLICATION HIGHLIGHT ON NEW INHIBITORS:

Millicell® µ-Migration Assay Kit

MMA205

EMD Millipore’s newly released MDMX Inhibitor, NSC207895 is shown to block the action of MDMX. The inhibitor is based on the important apoptotic regulation paper entitled, A small-molecule inhibitor of MDMX activates p53 and induces apoptosis.* apoptosis.*

14-3-3 Antagonist I, 2-5

Anti-Vacuole membrane protein 1 (VMP1) Anti-Vsp4/SKD1, clone 5E6.1

ABC66 MABC83

Wang et al. from Albany Medical College report that MCF-7 breast cancer cells treated with NSC207895 show activation of p53, which results in the elevated expression of several proapoptotic genes. They also showed that NSC207895 works additively with Nutlin-3a to activate p53 and diminish the viability of cancer cells. * Mol. Cancer Ther. (2011) 10: 69-79.

For published studies using this new product, visit: www.millipore.com For publications on using these small molecules, visit: www.emd4biosciences.com 4

Description

Anti-Endonuclease G

Small Molecules and Inhibitors 100081-5mg

14-3-3 Antagonist II, BV02

100082-10mg

Anaph phaase-Promotin ingg Co Complex In Inh hibitor, TA TAME

172104-50mg

EF24

324510-10mg

GSK-3β Inhibitor XXIV  Hdm2 E3 Ligase Inhibitor II, HLI373

361567-5mg 373226-5mg

Heat Shock Factor 1 Inhibitor, KRIBB11

385570-10mg

InSolution™ ER ERK In Inhibitor IIII, FR FR180204

328010-500µg

InSolution™ MG-132 in EtOH, ≥95% by HPLC

474788-10mg

InSolution™ STAT3 Inhibitor III, WP1066

573129-5mg

InSolution™ TGF-β RI Kinase Inhibitor VI, SB431542

616464-5mg

InSolution™ Y-27632 in DMSO

688002-1mg

MDMX Inhibitor, NSC207895

444158-10mg

MG-132, ≥95% by HPLC

474787-10mg

MKLP-2 Inhibitor, Paprotrain

512533-25mg

PICK1 PDZ Domain Inhibitor, FSC231

529531-10mg

SHIP1 Inhibitor, 3AC

565835-10mg

Stat3 Inhibitor XIII, C188-9

573128-10mg

TNP-470

654270-10mg

WP1130

681685-10mg

 

 VOLUME  VOL UME 2 I 2012

NEW PRODUCTS Cell Structur Structuree Description

Neuroscience Catalogue No.

Antibodies Anti-Abl interactor 1, clone 19C3.1 Anti-Alpha-(1,3)-fucosyltransferase 7 Anti-Arp2/3 complex, clone 13C9 Anti-CELSR1 Anti-Dynamin-1/2, clone Hudy-1 Anti-Girdin, C-terminus, clone 10E6.1 A asyep(tL1OX An nttii--LpyhsoyslpOhxoid-M (S)er507) Anti-Protein Regulator of Cytokinesis 1 (PRC1) Anti-T-cadherin (CDH13)

Catalogue No.

Antibodies MABT93 ABT117 MABT95 ABT119 MABT188 MABT100 0A7B-1T151027 ABT120 ABT121

Kits & Assays LentiBrite™ Paxillin-RFP Lentiviral Biosensor LentiBrite™ α-actinin-GFP Lentiviral Biosensor

Description

17-10155 17-10156

Anti-β III Tubulin, Biotin conjugate Anti-β III Tubulin, Cy3 Conjugate Anti-Ceruloplasmin (CP) Anti-FGF10 Anti-HtrA1, clone ME-1 Anti-MAP2, Alexa Fluor® 488 Conjugate A An nttii--M MA DPG2A,1Cy3 Conjugate Anti-NeuN Anti-Numb, clone 6G10.1 Anti-PCTAIRE-1 kinase, clone 9G10.1 Anti-PDE9A Antii-TTAR DNA binding protein-43, clone TAR5 2H4 Anti-VPAC2

AB15708B AB15708C3 ABN39 ABN44 MABC77 AB2290A4 ABA22B9202C236 ABN90 MABN281 MABN89 ABN32 MABN BN15 1500 AB2266

Small Molecules and Inhibitors γ-Secretase Inhibitor XXIV, BMS299897 GSK-3β Inhibitor XXIV 

Epigenetics & Gene Regulation Description

Catalogue No.

Antibodies Anti-Dnmt3l Anti-FTO, clone 5-2H10 Anti-GATA3, clone 3F1.1 Anti-HIF-1α, hydroxyproline (Pro402) Anti-hnRNP-P2, clone LHC7.15

ABD78 MABE227 MABE271 07-1585 MABE192

Anti-HpTGEKP motif Anti-LSD1 Anti-monomethyl-Histone H3 (Lys79) Anti-NFκB p65, CT Anti-phospho-Histone H3 (Ser28), clone HTA28 Anti-RNF168 Anti-SMN2, clone SMN-KH Anti-TAF3 Anti-trimethyl Histone H3 (Lys36) Anti-trimethyl-Histone H3 (Lys18) Anti-Tristetraprolin Anti-Wnt-1, clone 20C3.1 ChIPAb+™ HDAC1 ChIPAb+™ HDAC3 ChIPAb+™ JHDM1B ChIPAb+™ N-CoR

ABE319 ABE365 ABE213 ABE347 MABE76 ABE367 MABE230 07-1802 ABE305 ABE289 ABE285 MABS95 17-10199 17-10238 17-10264 17-10260

Small Molecules and Inhibitors HDAC Inhibitor XXIII, Tubastatin A HMTase Inhibitor V, UNC0224 MDMX Inhibitor, NSC207895 Prootei Pr ein n Me Meth thyl yltr tran ansf sfer eras asee In Inhi hibi bittor II II,, BI BIX X-0 -013 1338 38

382187-5mg 382193-5mg

NOX1 Inhibitor, ML171 PICK1 PDZ Domain Inhibitor, FSC231 β-Amyloid Oligomer Inhibitor, K01-162 Neurogenesis Enhancer, P7C3A20

565793-5mg 361567-5mg 492002-10mg 529531-10mg 200487-10mg 480744-5mg

Stem Cell Research Description

Catalogue No.

Antibodies Anti-Brn-2 (POU3F2), clone 8C4.2 Anti-DAZL Anti-DMP-1 (C-terminus), clone 8G10.3 Anti-Fibroblast-specific Protein 1 (S100A4) Anti-Musashi-2 Anti-NANOG, clone 7F7.1 Anti-Rfx6 Anti-SNAI1 (Snail homolog 1) RIPAb+™ PUM1 RIPAb+™ PUM2

MABD51 ABD31 MABD19 ABF32 MABE269 MABD24 ABD28 ABD38 03-242 03-241

Small Molecules and Inhibitors BMP Inhibitor II, DMH1 InSolution™ TGF-β RI Kinase Inhibitor VI, SB431543 InSolution™ Y-27632 in DMSO Neurogenesis Enhancer, P7C3A20

203646-5mg 616464-5mg 688002-1mg 480744-5mg

444158-10mg 5392 53 9212 12-2 -2mg mg

Assay Development Description Daptomycin, Streptomyces sp. Phos Ph osph phat atas asee In Inhi hibi bito torr Co Cock ckta tail il Se Sett II II,, Lyo yoph phil iliz ized ed Phosphatase Inhibitor Cocktail Set IV, Lyophilized

Catalogue No. 268320-5mg 5246 52 4636 36-1 -1SE SETT 524633-1mL

For published studies using this new product, visit: www.millipore.com For publications on using these small molecules, visit: www.emd4biosciences.com 5

 

 VOLUME  VOL UME 2 I 2012

NEW PRODUCTS Signaling Description

Catalogue No.

Antibodies Anti-Adipose Triglyceride Lipase (PNPL A2) Anti-CD2, clone RPA-2.10 Anti An ti-C -CD3 D3 ga gamm mmaa de delt ltaa (H (HMT MT33-2) 2),, clo clone ne Ha Ham2 m255-11 1157 57 Anti-Fibroblast-specific Protein 1 (S100A4) Anti-GATA3, clone 3F1.1 Anti-Grb10 α β, clone 12B11.1 Anti-GSK-3 Anti-murine T/cell receptor Vbeta 8.1 and 8.2, clone KJ16-133 Anti-Myeloid di dif fe ferentiation-2 (M (MD-2), cl clone IIIIC1 Anti-PD-1, clone RMP1-14 Anti-phospho-LRRK2 (Tyr1491) Anti-phospho-LRRK2 (Tyr1503) Anti-phospho-Mypt1 (Ser507) Anti-phospho-PDGFRα (  (TTyr754) Anti-phospho-PFKFB2 (Ser466) Anti-phospho-SRPK2 (Ser494) Anti-phospho-VEGFR2 (T (Tyr1212), cl clone 12 12A10.1 Anti-Plk3 Anti-RAS-Related protein Ral-A Anti-ROR gamma T, clone 6F3.1 Anti-SKAR Anti-STK33 Anti-Thioredoxin Reductase 2, clone 1C4 Anti-Tim3, clone 5D12 Anti-TLR3, clone 2B11.2 Anti-TLR6, clone 11C5.1 Anti-Wnt-1, clone 20C3.1 Anti-Wrch-1 Milli-Mark™ Anti-Tie2/TEK-PE, clone Ab33

Calbiochem®: Publish or Perish

Description

Catalogue No.

Kits & Assays ABD66 MABF83 MABF MA BF11 1166 ABF32 MABE271 07-2182 MABS77 MABF95 MABF117 04-1518 ABS108 ABS107 07-1507 ABS150 07-1531 07-1817 MABS191 ABS89 ABS223 MABF81 ABS157 ABS205 MABS179 MABF73 MABF72 MABF70 MABS95 ABS99 FCMAB404PE

Mouse Fibroblast Growth Factor-23 (FGF-23) ELISA Kit

EZMFGF23-43K

Small Molecules and Inhibitors BMP Inhibitor II, DMH1 Forskolin, Coleus forskohli i in DMSO GSK-3β Inhibitor XXIV 

203646-5mg 344282-5mg 361567-5mg

InhibitorSelect™ 96-Well Tyrosine Phosphatase Inhibitor Library IV Kinase and InSolution™ Src Inhibitor, PP1 InSolution™ Akt Inhibitor V, Triciribine InSolution™ PP1 Analog InSolution™ PTP1B Inhibitor InSolution™ RSK Inhibitor, SL0101 InSolution™ STAT3 In Inhibitor VI VI, S3 S3I-201 InSolution™ TGF-β RI Kinase Inhibitor VI, SB431544 InSolution™ U0126 InSolution™ Y-27632 in DMSO Insulin Expression Inducer, BRD7389 PIP3 Antagonist, PITenin-7 PIP3 Antagonist, PITenin-7 RAF Kinase Inhibitor VII SHIP1 Inhibitor, 3AC Wnt Antagonist V

539747-1EA 539571-5mg 124038-2mg 529605-1mg 539749-2mg 559292-500µg 573130-5mg 616464-5mg 662009-5mg 688002-1mg 203709-10mg 524618-10mg 524618-10mg 553018-5mg 565835-10mg 681677-10mg

For published studies using this new product, visit: www.millipore.com For publications on using these small molecules, visit: www.emd4biosciences.com

FEATURED CALBIOCHEM® PRODUCTS SMALL MOLECULE LIBRARIES & PA PATHWA THWAY Y PANELS

InhibitorSelect™ 96-Well Tyrosine Kinase

Enzyme Family

Number of Inhibitors in Library

Receptor Tyrosine Kinase (RTK)

37

and Phosphatase Inhibitor Library IV 

Tyrosine Kinase (TK) Tyrosine Kinase-like (TKL)

27 6

PKA, PKG, PKC families (AGC)

1

(Catalogue No. 539747)

CDK, MAPK, GSK-3, CLK families (CMGC)

3

Receptor Tyrosine Phosphatase (RTP)

1

Tyrosine Phosphatase (TP)

8

This library consists of 83 pharmacologically active, well-documented, cell-permeable, potent, and reversible protein kinase and phosphatase inhibitors; the majority of kinase inhibitors are are ATP-competitive. ATP-competitive. They are supplied in a convenient 96-well plate format at a concentration

InhibitorSelect™ Library Advantages: •

of 10 mM in DMSO. Use this library to advance advance target identification in drug discovery, biochemical pathway

•

analysis, screening new protein kinases/phosphatases, kinases/phosphatases, high content screening, and more.

6

•

Well characterized with strict quality control for reproducible results Structurally diverse compounds are useful to Structurally distinguish off-target effects High potency to yield unambiguous data

 

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EMD Millipore, the M logo, Milli-Mark, ChIPAb+, RIPAb+, EpiQuant, LentiBrite, InSolution, and InhibitorSelect are trademarks of Merck KGaA, Darmstadt, Germany. MILLIPLEX, Chemicon, and Upstate are registered trademarks of Merck KGaA, Darmstadt, Germany. Lit No. No. PR4774EN00 PR4774EN00 LS-SBU-12-06173 Printed in the USA. 4/2012 © 2012 EMD Millipore Millipore Corporation, Billerica, MA USA. All rights rights reserved. reserved.