of x

Recommendations For The Health Monitoring of Mouse, Rat, Hamster, Guineapig and Rabbit Breeding Colonies

Published on 2 weeks ago | Categories: Documents | Downloads: 0 | Comments: 0

Comments

Content

 

Recomm Reco mmen enda dati tion ons s for for the the he heal alth th moni monito tori ring ng of  mous mo use, e, ra rat, t, ha hams mste ter, r, guin guinea eapi pig g and and rabb rabbit it breeding bree ding colonies colonies Report of the Federation of European Laboratory Animal Science Ass As socia ciation ions (FELA FELAS SA) Wo Workin king Group roup on Anim Anima al Healt ealth h accep ac cepted ted by th the e FELASABo FELASABoard ard of Managem Management ent Novemb November er 199 1992 2 FELASAWorki FELASAWo rking ng Gro Group up on Ani Animal mal Hea Health lth:: V. Kra Kraft ft (GV (GV-SO -SOLAS LAS)) C Conv onvene ener, r, A. A. De Deen eny y (LAS (LASA) A) Se Secr cret etar ary, y, H. M. Bl Blan anch chet et (S (SFE FEA) A),, R. Bo Boot ot (N (NVP VP), ), A. K. Hans Hansen en (S (Sca cand nd-L -LAS AS), ), A. He Hem m (S (Sca cand nd-L -LAS) AS),, H. va van n He Herc rck k (N (NVP VP), ), I. Kun Kunst styr yr (G (GVV-SO SOLA LAS) S),, G. Mi Mili lite te (A (AIS ISAL AL), ),   J .   R. Needh Needham am (L (LASA ASA), ), W.   Nickl Nicklas as (GV(GV-SOL SOLAS) AS),, A. Per Perrot rot (SF (SFEA) EA),, C. Reh Rehbin binder der (St (Stand and-LA -LAS), S),  Y. Richard (SFEA) & G. De Vroey (BCLAS) FE FELA LASA SA,, BCM BCM Bo Box x 2989 2989,, Lon London don WC WC1 1 N 3XX 3XX,, UK

Contents Preamble 1. Gen Genera erall conside considerat ration ionss 2. Freq Frequen uency cy of mo moni nito tori ring ng and and sa samp mple le si size ze 3. Viral Viral infecti infections ons 3. 3.1 1 Mice Mice 3. 3.2 2 Rats Rats 3.3 Hamste Hamsters rs 3.4 Guineap Guineapigs igs 3.5 Rabbit Rabbitss 4 .   Bacter Bacterial ial,, mycopl mycoplasm asmas as and funga fungall infect infection ionss 4.1 Method Methodolo ology gy 4.2 Sample Sampless to be investi investigate gated d 4. 4.3 3 Mice Mice an and d ra rats ts 4.4 Hamste Hamsters rs 4.5 Guineap Guineapigs igs 4.6 Rabbit Rabbitss 5. Parasitolog Parasitology y 5.1 Methodology Methodology 5.2 Report Report of result resultss 5. 5.3 3 Test Test sche schedu dule le in mou mouse se,, ra rat, t, ha hams mste terr and and guine guineapi apig g br breed eedin ing g un unit itss 5. 5.4 4 Test Test sche schedu dule le in rabb rabbit it br bree eedi ding ng un unit itss 6. Pathol Pathology ogy 7. App Appen endi dix x I-Nec I-Necro rops psy y pr proc ocedu edure ress 8. Appe Append ndix ix II-G II-Gui uide deli line ness fo forr the the Use of the the FELA FELASA SA Appr Approv oved ed Health Hea lth Mon Monito itorin ring g Report Report 9. Append Appendix ix III- Abbrev Abbreviat iation ionss

2 2

3 3

3 4

5

5 6 6 6 6 6

7 8 8 9 9 9 9

10 10 10 11 12

laboratory   Animals   (1994) (1994) 28, 1-12

 

2

Preamble The he The healt alth h of an an an anim imal al is alway alwayss at risk  risk  from a variety variety of infecti infection ons. s. Such Such infecti infection onss may ma y be inapp inapparen arentt or at least least not not made apparent appa rent by gross and obvious lesions. lesions. Clini Clinical cal diseas disease e may thus thus not not be observe observed d until unt il the animal animal is stressed stressed,, for exam example ple by an experimental experimental procedure. procedure. There The re is overwhelm overwhelming ing evidence evidence that infect inf ection ionss in laborato laboratory ry anima animals ls can often often influence influe nce the outcom outcome e of experim experiments. ents. Depen De pendin ding g upon upon the sp specif ecific ic infect infection ion,, a variety of biologic biological al parameters parameters may be affected, affecte d, including behaviour, behaviour, growth growth rate, relative organ organ weigh weights, ts, immune immune response response and tumou tumourr develo developm pment ent.. Subtl Subtle e or overt overt infections infect ions can also lead t9 contamin contamination ation of biol biolog ogical ical materi materials als,, tissue tissue culture cultures, s, cellcelllines, lin es, transpl transplant antabl able e tumou tumours rs and biobiological log ical produ products. cts. All All infectio infection, n, appare apparent nt or  inapp ina pparen arent, t, is likely likely to increas increase e biolog biologica icall variability. Some Som e laboratory laboratory animal animal diseases diseases are zoonotic. Forr all these Fo these reason reasons, s, a laborator laboratory y anima animall health hea lth monit monitori oring ng progra programm mme e is of vital importan imp ortance, ce, decreasing decreasing the risk of zoonoses zoonoses an and d adding adding to the reliabi reliability lity and reproreproducibility duci bility of research research data. This Th is report report propos proposes es a schem scheme e for health health monitorin mo nitoring g of laborato laboratory ry animal animal breeding breeding co colon lonies ies with the intent intention ion of harm harmon onizin izing g  proc  pr oced edur ures es amon among g th thos ose e co coun untri tries es as asso so-ciated with FELAS FELASA. A. It is the intent intention ion of FEL FELAS ASA Ato keep these recommenda recommendations tions under under periodical periodical review rev iew and to publis publish h amend amendm ments ents as necessary.

1. Genera Generall consid considera eratio tions ns 1.1 1.1 These These recomme recommend ndatio ations ns constit constitute ute a commo com mon n appro approach ach for all breeders breeders of  laboratory labora tory animals. animals. Actual Actual practice may may differ from these these recomme recommendatio ndations ns in variouss ways variou ways dependin dependingon gon localcircum local circumstance stancess - for examp example le colon colony y size, size, regional regional  prev  pr eval alen ence ce of specif specific ic or orga gani nism sms, s, in inten tende ded d use of progen progeny, y, or existe existence nce of nation national al monitorin mo nitoring g schemes. schemes. Additiona Additionall investiinvestigationss may be deemed gation deemed neces necessary: sary: should should

FELAS FEL ASA A heal health th mo monito nitoring ring

reco recomm mmenda endation tions s

these indica these indicate te the presen presence ce of an an agent agent which wh ich,, althou although gh not not listed listed in these recomrecommend me ndatio ations, ns, is suspecte suspected d of being being impo importan rtant, t, thatt agent tha agent should should be mentio mentioned ned in su succe ccessssive report reportss and treated treated as are listed listed agents agents.. 1.2 These recomme recommendati ndations ons are intended intended for all breedi breeding ng co colon lonies ies of mice, mice, rats, rats, hamsters, ham sters, guineapig guineapigss and rabbits. rabbits. 1. 1.3 3 The term 'breedi 'breeding ng unit' unit' is here here underunderstood to describe a self-contained unit, which whi ch could be considered considered a microbiolog microbiological ical en entit tity y no matte matterr how how many many strain strainss or  specie spe ciess are kept kept in it (see (see 1.10, 1.10, 1. 1.11 11). ). 1. 1.4 4 The The existen existence ce of detai detailed led writte written n proprocedures cedu res - Standard Standard Operatin Operating g Procedures Procedures (SOPs) (SO Ps)with within in monitori monitoring ng laboratories laboratories is expected. expe cted. Such SOPs SOPs must must be available available on request. I.S Monitorin Monitoring g laboratories laboratories should should follow principles of Good Laborator Laboratory y Practice} Practice} the th e   principles where wh ere applicable. applicable. 1.6 Monitorin Monitoring g laboratories laboratories should should  parti  pa rtici cipa pate te in a Quali Quality ty Ass ssur uran ance ce Pr Proogramme. 1. 1.7 7 It should should be emph emphasiz asized ed that that negativ negative e results res ults mean mean only only that the presence presence of the microorg mic roorganism anismss monitore monitored d has not been demon dem onstra strated ted in the anima animals ls screene screened d by the test(s) test(s) used. used. The The results results are no nott necess nec essaril arily y a reflecti reflection on of the the status status of all all the anima animals ls in the breedi breeding ng unit. unit. 1. 1.8 8 An agen agentt must must be declare declared d as present present if  it is iden identif tified ied or antib antibod odie iess to it are detecte det ected d in one or more more of the the anima animals ls screen scr eened. ed. The results results must contin continue ue to be reporte rep orted d as positiv positive e at subseq subsequen uentt screen screenss until un til a subse subsequ quent ent test test is negat negative ive after, after, for  example, exam ple, the organism organism has been eradicated eradicated  by mea eans ns of rederi rederiva vatio tion n or re resto stock ckin ing g by animals anim als from external external sources. sources. Howev Ho wever, er, agents agents known known to be present need nee d not be monito monitored red at subseq subsequen uentt screen scr eenss provid provided ed that they they are declared declared in the health health report report.. 1. 1.9 9 The The presen presence ce of antibo antibodie diess in a colony colony is only an indica indicator tor of infec infectio tion. n. Its signisignificance ficanc e can be elucidated elucidated using using method methodss other than serological serological method methods. s.

 

FELASA FEL ASA hea health lth

monito monitorin ring g

3

rec recom ommen mendat dation ions s

1.1 .10 0 As the the qu ques estio tion n of st stra rain in sp spec ecif ific icit ity y of  infe infect ctio ions ns is no nott full fully y under underst stoo ood, d, in anim animal al units units conta containi ining ng mo more re than than on one e strain strain of the sam same e sp speci ecies, es, the strai strains ns must must be sc scree reene ned d su succ cces essi sive vely ly and and each each stra strain in sh shou ould ld be monit onitor ored ed at leas leastt once once a yea year. r. The

freq freque uenc ncy y of monit onitor orin ing g for for the un unit it sh shou ould ld co com mpl ply y wi with th th the e ge gene nera rall test test sche schedu dule le.. 1.1 .11 1 If a uni unitt cont contai ains ns more ore than than one an anim imal al sp spec ecie ies, s, each each sp spec ecie iess must must be sc scre reen ened ed sepa separa rate tely ly,, acco accord rdin ing g to th the e test test schedule.

2. Freq Freque uenc ncy y of monit monitor orin ing g an and d samp sample le size Tabl Table e 2a

Mous Mouse, e,

ra rat, t, ha hams mste terr

an and d guin guinea eapi pig g

br bree eedi ding ng

 

Sample Sam ple size

un unit its s

Testingfanimal

Sampling frequency

Age

No. animals

Every 3 months

Weanling

~2

10-14   weeks   (young (youn g adult adults) s)

~4

>6 mo mont nth hs (r (ree- ~4

Serology

Bacteriology

Parasitology

Pathology

+

+

+

+

+

+

+

+

+

+

+

tired breeders) breeders) Tabl Table e 2b

Ra Rabb bbit it br bree eedi ding ng

unit units s

Sample size Sampling frequency Every 6 mont months hs

Testing/animal

Age  

No. animals

Serology

Bacteriology

Parasitology

Pathology

~4

+

+

+

+

>6 mo mont nth hs (r (ree- ~4 tired breeders) breeders)

+

+

+

+

12-14   weeks

 

(yo (young ung adults adults))

3. Vira Virall in infe fect ctio ions ns 3. 3.1 1 Mice Mice Ta Tabl ble e 3a No.

Vira Virall in infe fect ctio ion ns

to b be e s se erolo rologi gica call lly y

monit onito ored red

in   mouse   bree breedi ding ng

unit units s

 Antigens

Sui Suitab table le tes testt method methods s (al (alpha phabet betica ical) l)

Mi Minu nute te viru virus s of mi mice ce (MVM) (MVM) Mouse Mou se hepati hepatitis tis virus virus (MHV)

ELISA, HI, HI, IFA ELISA, IFA

3 4

Pneu Pneumo moni nia a

ELISA, HI, HI, IFA

Reoviru Reo virus s type type 3 (Reo (Reo3) 3)

ELISA, IFA

S

Sendaii virus Senda

ELISA, HI, HI, IFA

6

Theiler The iler's 's murine murine enceph encephalo alomye myeliti litis s

7

** **Ectr Ectrome omelia lia virus virus ** Hantaviruses

1 2

8

viru virus s of mi mice ce (PVM (PVM))

viru virus s (TM (TMEV) EV)

ELI ELISA, SA, HI, lFA ELISA,IFA ELISA, HI, HI, IFA

9 10

**L **Lact actic ic

11

** **Mou Mouse se

adenov adenovirus irus (MA (MAd) d)

ELISA, IFA

12

**Mous **M ouse e

rot rotavi avirus rus (ED (EDIM) IM)

ELISA, IFA

13 14

**Mo **Mous use e

K viru virus s (K)

ELISA, HI

**Mo **Mous use e

poly polyom oma a

ELISA, HI, HI, IFA

1S

**Mo **Mous use e

th thym ymic ic virus virus (MTV (MTV))

ELISA, IFA

16

**Mous **M ouse e

cytomeg cytomegalo aloviru virus s

ELISA, IFA

deh dehydr ydroge ogenas nase e

** **Lym Lympho phocyt cytic ic

virus virus (LDV (LDV))

cho choriom riomeni eningi ngitis tis

viru virus s (LC (LCM) M)

virus virus (MC (MCMV) MV)

LDH plasma plasma tes testt ELISA, IFA

**Viruses **Viru ses for which evidence exists exists of rare inf infectio ections ns in Europe European an mouse colonies. colonies. Howe However, ver, they shoul should d be teste tested d in rederived rederived or res restoc tocked ked colonies colonies and in anima animals ls pri prior or to use in Mouse Mouse Antib Antibody ody Pro Produc ductio tion n (MAP) (MAP) te testi sting ng

 

 

4

FELASA FEL ASA hea health lth

monito monitorin ring g

recom recommen mendat dation ions s

Sampling Sampl ing frequency frequency

Equivocal Equivoc al or unexpected unexpected positive positive serologica log icall test results results must must be confirm confirmed ed by an alternat alternative ive test test method ethod and/or and/or repeate repeated d investigation. An agent agent must must be declared declared as presen presentt if antib antibod odie iess to it are are detecte detected d in one one

Every three Every three months months:: Antigen Antigen numbe numbers rs   1-6. Once On ce a year: Antigen Antigen number numberss 7-10. 7-10. Sample Sam ple size size A mi minim nimum um of 8 indiv individu idual al anim animal sera sera

or more more anima animals ls screene screened. d. The The results results must mu st contin continue ue to be repo reported rted as posipositive tiv e at succes successiv sive e screen screenss until until the agent age nt has been eradicat eradicated ed by means means of  e.g.. rederiv e.g rederivatio ation, n, restock restocking ing.. An agent agent report rep orted ed as presen presentt need need not not be monimonitored tor ed at subsequ subsequent ent screen screenss but it must mu st be declare declared d in subseq subsequen uentt reports.

(not pooledl pooledl from mice mice randomly randomly sam sampled pled from each breeding unit. Tabl Table e 3b

Sa Samp mple le si size ze an and d ag ages es-m -mic ice e

 Age

No. of ani animal mals s

10-14   weeks weeks (yo (young ung adults) adults)

;;::4

>6 months (retir (retired ed breeders) breeders)

;::4

3. 3.2 2 Rats Rats Ta Tabl ble e 3c

Vira Virall in infe fect ctio ion ns

to be monito nitore red d

sero serolo logi gica call lly y

in   rat   bree breedi ding ng

unit units s

No.

 Antigens

Sui Suitab table le test test method methods s (alphab (alphabeti etical cal))

1 2

Hantaan Hantaa n viru virus s Kilha Kilham m ra ratt virus virus (KRV) (KRV)

ELISA, HI, IFA HI, (ELISA IFA)··

3

Pneu Pneumo moni nia a

ELISA, HI, HI, IFA

4

Reovir Reo virus us type type 3 (R (Reo eo 3)

5

Sendaii virus Senda

6

Sialod Sialodacr acryoa yoaden denitis itis

7

Theile The iler's r's murine murine enceph encephalo alomye myeliti litis s

8

Toolan Too lan (H(H-1) 1)

virus virus of mi mice ce (PV (PVM) M)

ELISA, IFA ELISA, HI, HI, IFA

(SD (SDA)/ A)/Rat Rat coro coronav naviru irus s

ELISA, IFA

(ReV) (ReV)

ELISA, HI, HI, IFA

viru virus s (TM (TMEV) EV)

HI, (ELISA (ELISA,, IFA)··

**I **In n the case case of rat par parvov voviru iruses ses (KRV, H-1) H-1) a anti ntibod bodies ies to these these viruse viruses s may cross cross-re -react act w with ith the ant antige igens ns in IFA and and ELI ELISA. SA. However, Howe ver, these infe infection ctions s can be differenti differentiated ated by spe specific cific HI tests

Equivocall or unexpected Equivoca unexpected serological serological testt results tes results must must be confi confirm rmed ed by an alterna alte rnativ tive e test test method ethod and/or and/or repeat repeated ed investigation. . An agent agent must must be declare declared d as present present if  an antib tibod odie iess to it are are de dete tecte cted d in one one or mor more e anim ani mals screene screened. d. The The results results must must continu con tinue e to be reported reported as positi positive ve at succes suc cessiv sive e screens screens until until the agent agent has been been eradicated eradic ated by means means of e.g. rederivation, rederivation, restoc res tockin king. g. An agent agent report reported ed as present present need nee d not not be monit monitore ored d at subseq subsequen uentt scre screen enss but but it mus ustt be decla declare red d in subseque subs equent nt reports. reports.

Sampling Sampl ing frequency frequency Every Eve ry three three months: months: Antigen Antigen numbers numbers   1-8. Sample Sam ple size size A mini minimu mum m of 8 individ individual ual anim animal sera sera (not pooled) pooled) from rats randomly randomly sampled sampled from each breeding unit. Tabl Table e 3d

Sa Samp mple le si size ze and and ag ages es-r -rat ats s

 Age

No. of ani animal mals s

weeks (yo (young ung adults) adults) 10-14   weeks

>6

months mont hs (retir (retired ed

bre breede eders) rs)

 

FELASA FEL ASA hea health lth

monito monitorin ring g

rec recom ommen mendat dation ions s

5

3.3 Hamste Hamsters rs Ta Tabl ble e 3e No.

Vira Virall in infe fect ctio ion ns

to b be e m mon onit itor ore ed

sero serolo logi gica call lly y

in   hamster    bree breedi ding ng

unit units s

Sui Suitab table le test test method methods s (al (alpha phabet betica ical) l)

 Antigens

1

Lympho Lym phocyti cytic c

2

Pneu Pneumo moni nia a

3

Reovir Reo virus us type type 3 (Re (Re03) 03)

ELISA, IFA

4

Sendai virus Sendai Simian Sim ian viru virus s 5 (SV5 (SV5))

ELISA, HI, HI, IFA ELISA, IFA

5

cho chorio riomen mening ingitis itis

viru virus s (L (LCM) CM)

ELISA, IFA

virus virus of mi mice ce (PVM (PVM))

ElI ElISA, SA, HI, IFA

Equivocal or unexpected positive Equivocal serolog ser ological ical test results results must must be confirm confirmed ed  by an alter alterna nativ tive e test test method ethod an and/o d/orr by repeated repea ted investigation. investigation. An agent agent must be declar declared ed as pres presen entt if  an antib tibod odies ies to it are are detecte detected d in on one e or m mor ore e of the the anima animals ls screen screened. ed. The The results mu must st co conti ntinu nue e to be report reported ed as positi positive ve at subs subseq eque uent nt scree screens ns until the ag agen entt has has  been  be en erad eradica icated ted by mea eans ns of e.g. re rede deririvation or restock restocking ing.. An agent agent reported reported as  pres  presen entt need need not not be monit monitor ored ed at subseq sub sequen uentt screens screens but but it must be decla declared red in subsequen subsequentt reports reports..

Sampling frequency Sampling frequency Every Ev ery three three months months:: Antige Antigen n number numberss   1-5. Sample size Sample size A mini minim mum of 8 indivi individu dual al an anim imal al sera sera lnot pooled) pooled) from hamster hamsterss random randomly ly sampled sam pled from each each breedin breeding g unit. unit. Tabl Table e 3f

Sa Samp mple le si size ze and and ag ages es in ha hams mste ters rs

 Age

No. of ani animal mals s

weeks s (yo (young ung adults) adults) 10-14   week

~4

>6 months months (retir (retired ed

bre breede eders) rs)

3.4 Guineap Guineapigs igs Ta Tabl ble e 3g

Vira Virall

in inffecti ection ons s

to b be e m mon onit itor ored ed

sero serolo log gica ically lly

in   guineapig    bree breedi ding ng

No.

 Antigens

1

Guineapig

2

Lympho Lym phocyt cytic ic

3

Pneu Pneumo moni nia a virus virus of mi mice ce (PV (PVM) M)

ELISA, HI, HI, IFA

4

Reovi Reo vi rus ty type pe 3 (Re03) (Re03)

ELISA, IFA

5 6

Sendaii virus Senda

ELISA, HI, HI, IFA

Simian Sim ian viru virus s 5 (SVS (SVS))

ELISA, IFA

unit units s

Sui Suitab table le tes testt metho methods ds (al (alpha phabet betica ical) l) adenovirus

ELISA, IFA ELISA, IFA

(GpAd)

cho choriom riomeni eningi ngitis tis

virus virus (LC (LCM) M)

Equivocal or unexpected Equivocal unexpected positive serologi sero logical cal test results results must must be confirm confirmed ed  by an alter alterna nativ tive e test test method ethod an and/o d/orr by repeated repea ted investigation. investigation. An agen agentt must be declar declared ed as pres presen entt if  an antib tibod odies ies to it are are detec detected ted in on one e or m mor ore e of the the anima animals ls screen screened. ed. Th The e res results ults must must co conti ntinu nue e to be repo reporte rted d as p pos ositiv itive e at subse subsequ quen entt scree screens ns un until til the ag agen entt has has  been  be en erad eradica icated ted by mea eans ns of e.g. e.g. re rede deririvation vati on or restock restocking. ing. An agent agent reported reported as  pres  presen entt need need no nott be monito onitore red d at subs subseq eque uent nt scree screens ns bu butt it must be de decla clare red d in sub subseq sequen uentt reports reports..

Sampling Sampl ing frequency frequency Every Ev ery three three months months:: Antige Antigen n number numberss   1-6.

Sample Sam ple size size A minim inimum of 8 indivi individu dual al an anim imal al sera sera (not pooled) pooled) from guineapigs randomly randomly sampled sam pled from each each breedin breeding g unit. unit. Ta Tabl ble e 3h

Samp Sample le siz size e and ages ages-g -gui uine neap apig igs s

 Age

No. of ani animal mals s

weeks (yo (young ung adults) adults) 10-14   weeks

~4

>  6 months months (retir (retired ed bre breede eders) rs)

~4

 

FElASA FElASA hea health lth

6

monito monitorin ring g

recom recommen mendat dation ions s

3.5 Rabbits Rabbits Ta Tabl ble e 3i

Vira Virall

in infe fect ctio ions ns

to b be e m mon onit ito ored red

sero serolo log gical ically ly

in   rabbit    bre breed edin ing g

un unit its s

No. No.

 Antigens

Sui Suitab table le tes testt metho methods ds (al (alpha phabet betica ical) l)

1

Pneu Pneumo moni nia a virus virus of mi mice ce (PVM) (PVM)

ElI ElISA, SA, HI, HI, IF IFA A

2

Rabbit Rab bit haemor haemorrha rhagic gic diseas disease e viru virus s (RH (RHDV) DV) Rabbit pox viru Rabbit virus s (my (myxom xomato atosis) sis)

ELISA

Rabbit rotavi Rabbit rotavirus rus Sendaii virus Senda

ELISA, IFA

S 6

Simian Sim ian viru virus s 5 (SVS) (SVS)

3 4

ELISA, IFA ELISA, HI, HI, IFA ElI ElISA, SA, IFA

Equivocal or unexpected Equivocal unexpected positive serolog ser ological ical test results results must must be confirm confirmed ed  by an alter alterna nativ tive e test test method ethod an and/o d/orr by repeated repea ted investigation. investigation. An agent agent must must be declare declared d as presen presentt   if  an antib tibod odies ies to it are are detecte detected d in one or more more of the an anim imals als scre screen ened ed.. The The re resu sults lts must must co conti ntinu nue e to be repo reporte rted d as po posit sitive ive at

addition to non-selec addition non-selective tive media media for  routine rou tine and special special or confirm confirmato atory ry investiinvestigations. Aerob Ae robic ic culture culture conditio conditions ns are sufficient sufficient for most most bacteri bacteria. a. Wher here e possible possible,, identifica identification tion of micro micro-organi org anism smss should should procee proceed d to the specific specific name nam e e.g. e.g.  Paste  Pasteurella urella pneumotropic pneumotropica a   or 

subs subseq eque uent nt scree screens ns until until the ag agen entt has has  been erad  been eradica icated ted by mea eans ns of e.g. re rede deririvation vati on or restoc restocking king.. An agen agentt reporte reported d as  pres  presen entt need need not not be monit monitor ored ed at subs subseq eque uent nt scre screen enss but but it must ust be decla declare red d in subsequent seque nt reports.

 Mycoplasma pulmonis.

Sampling Sampl ing frequency frequency Every Ev ery six months: months: Antige Antigen n num number berss   1-6.

Sample Sam ple size size A minimu inimum m of 8 indivi individu dual al an anim imal al sera sera (nott pooled) (no pooled) from rabbits rabbits ran random domly ly sam sampled pled from fro m each each breedi breeding ng unit. unit. Ta Tabl ble e 3j

Samp Sample le si size ze a and nd ages ages-r -rab abbi bits ts No. of ani animal mals s

 Age 12-14   weeks weeks (yo (young ung adu adults lts)) >6 mo mont nths hs (r (ret etire ired d

;;l:4

bree breede ders rs))

4. Ba Bact cter eria ial, l, myco mycopl plas asma mall and and fung fungal al infections 4.1 Method Methodolo ology gy 4.1.1 Cultur 4.1.1 Cultural al method methodss   Bacteriological investiga inve stigation tionss must must always always include include non non-selectiv sele ctive e media media,, e.g. e.g. bloo blood d aga agar. r. Se Select lective ive an and d en enric riche hed d media media must be use used d   in

4.1.2 Serolo 4.1.2 Serologic gical al method methodss   Serological method ethodss ex exist ist fo forr the detec detectio tion n of  antibod anti bodies ies to various various bacteria bacteriall pathoge pathogens, ns, e.g.   Bacillus Bacillus piliformis, piliformis,   mycoplasm mycoplasmas as and  Leptospira   spp. Trepon Tre ponema ema cunicul cuniculii   in rabbit rabbitss may be monitored mon itored using   T. pallidum   or cardiolipin cardiolipin antigens. certain 4.1.3 Patholo 4.1.3 Pathologic gical al method methodss   In certain cases, cas es, e.g. CAR CAR bacillos bacillosis, is, histopat histopatholo hology gy may be the only only suita suitable ble method ethod of  detection.

4. 4.2 2 Sa Samp mples les to be inve invest stig igat ated  ed  Sa Samp mples les from the followin following g organs organs must must be cultured:: nasal turbinates/nasop cultured turbinates/nasopharyn harynx, x, trachea trac hea,, prepuc prepuce/v e/vagin agina, a, caecum caecum.. In addiaddition, tio n, seru serum m is sample sampled d fo forr the detec detectio tion n of  antibod anti bodies ies to  Bacillus Bacillus piliformis piliformis,,   myco plasm  pla smas as an and d  Leptospira   spp.

4. 4.3 3 Bact Bacter eria ial, l, myco mycopl plas asma mall an and d funga fungal  l  infe infect ctio ions ns to be moni monitor tored  ed    in   mi mice ce an and  d  rats  Bordetella bronchiseptica (428 280j0j-m mouse ouse Citrobacter Citro bacter freundii freundii   (4 Corynebacter Coryn ebacterium ium kutscheri kutscheri

only only

 

FElASA   health health mon monitor itoring ing



 Leptospira (icterohaemorrhagiae, ballum, canicol cani cola, a, hebdoma hebdomadis dis)) -   serology spp.- culti cultiva vatio tion n in ca case sess o of  f   Mycoplasma   spp. positi  po sitive ve sero serolog logy y  Pasteurella   spp. Salmonellae Streptobacill Strept obacillus us moniliformi moniliformiss Streptocci-,B-haem Streptocci-,B-h aemolytic olytic (exce (except pt D group) (designation (design ation of Lancefie Lancefield ld Group, Group, if   possi  po ssible ble)) Streptococcus Strept ococcus pneumoniae pneumoniae Tyzzer Tyz zer's 's disease disease (clinical (clinical disease/ disease/  patho  pa tholog logica icall lesion lesions/ s/ sero serolog logy** y**)) *To be screened only once per year  **Re **Results sults of serology serology are curren currently tly controcontroversial.

4.3.1 Additi 4.3.1 Additiona onall consid considera eratio tions ns   Examples of additio additional nal micro microorg organi anism smss to be monitored -when -whe n assoc associat iated ed with with lesion lesions, s, -whe -w hen n as asso socia ciated ted w with ith clinic clinical al signs signs of  of  disease, -whe -w hen n there there is evide evidenc nce e of pertu perturb rbati ation on of   phys  ph ysiol iolog ogica icall param paramete eters rs or bree breedin ding g  perf  pe rform orman ance ce,, -whe -w hen n using using spo sponta ntane neou ously sly imm immunounodeficientt animals. deficien animals. CAR bacillus CAR bacillus Derma Derm a tophytes tophytes  Escherichia coli  Klebsiella pneumoniae/oxytoca  Pneumocystis carinii  Proteus   spp.

 Pseudomonas aeruginosa Staphyl Stap hylococ ococcus cus aureus aureus Yersinia Yersi nia pseudotuberc pseudotuberculosis ulosis An agen agentt must ust be declar declared ed as pres presen entt if it is identif identified ied in one one or more more of th the e an anim imals als scre screen ened ed.. The The resu results lts must ust co conti ntinu nue e to be reporte rep orted d as positive positive at subseq subsequen uentt scr screen eenss until un til the ag agen entt has has been been erad eradica icated ted by means me ans of e.g. e.g. rederiv rederivatio ation n or resto restocki cking. ng. An agent agent repo reporte rted d as prese present nt need need no nott be monito onitore red d at subseq subseque uent nt scre screen enss bu butt it must mu st be declare declared d in subseq subsequen uentt reports. reports.

Sampling Sampl ing frequency frequency Every three months. Every months.

 

recomm recommend endatio ations ns

7

Sample Sam ple size size Ten animals Ten animals random randomly ly sample sampled d from each each  bree  breedin ding g unit. unit. Ta Tabl ble e 4a

Sa Sam mpl ple e size size a and nd ages ages-m -mic ice e

 Age

an and d rats rats

No No.. of animals animals

Weanlings 10-14   weeks weeks (youn (young g adults)

>6 mon months ths (retired (retired bre breede eders) rs)

4. 4.4 4 Bact Bacter eria iall an and d fu fung ngal al in infe fecti ctions ons   to   be moni mo nito tore red d in hams hamste ters rs  Bordetella bronchiseptica  Pasteurella   spp. Salmonellae Tyzzer's Tyzz er's disease (clinical (clinical disease/patholo disease/pathological gical lesions/ serology*) *Results of serology *Results serology are curren currently tly controcontroversial.

4.4.1 Additi 4.4.1 Additiona onall consider considerati ations ons   Examples of additio additional nal microo microorga rganism nismss to be monitored -whe -w hen n as assoc sociat iated ed with with lesions lesions,, - when when as assoc sociat iated ed with clinic clinical al signs signs of  disease, - when when there there is evide evidenc nce e of pertu perturb rbati ation on of   physi  ph ysiolo ologic gical al para param meters eters or bree breedin ding g  perfo  pe rform rman ance ce,, -when -wh en using using sponta spontaneo neously usly imm immuno uno-deficientt animals. deficien animals. Clostridium   spp. Dermatophytes  Escherichia coli  Klebsiella pneumoniae/oxytoca  Proteus   spp.  Pseudomonas aeruginosa Staphyl Sta phylococ ococcus cus aureus aureus An age agent nt mus ustt be declar declared ed as prese present nt if it is identif identified ied in one one or more more of the an anim imals als scre screen ened ed.. The The re resul sults ts mus ustt co conti ntinu nue e to be reporte rep orted d as positiv positive e at subseq subsequen uentt screen screenss until un til the ag agen entt has has been been erad eradica icated ted by means of e.g. means e.g. rederiv rederivatio ation n or restockin restocking. g. An agen agentt re repo porte rted d as pres presen entt need need not not be

 

8

 

FELASA FELA SA healt health h moni monitorin toring g

monitor monit ored ed at subse subsequ quen entt scre screen enss but but it must mu st be declare declared d in subseq subsequen uentt reports reports..

Sampling Sampli ng frequen frequency cy Every three three months. months.

Sample size Sample size Ten anima animals ls random randomly ly sampled sampled from each  breed  bre eding ing unit. unit. Table   4b   Sample Sample size and and ages-hams ages-hamsters ters  Age

No. No. of animals animals

Weanlings 10-14   weeks weeks (young (young adu adults) lts)

;;:4

>6 months (retired breeders)

;;:4

recom recommen mendatio dations ns

 Escherichia coli  Klebsiella pneumoniae/oxytoca  Proteus   spp.  Pseudomonas aeruginosa Staphyl Stap hylococ ococcus cus aureus aureus  An   ag agen entt mus ustt be declar declared ed as prese present nt if 

it is iden identif tified ied in one or more more of the the animals anim als screene screened. d. The results results must must concontinue tin ue to be report reported ed as posi positiv tive e at subseq sub seque uent nt scre screen enss un until til the ag agen entt has has  been  be en erad eradica icated ted by mean meanss of e.g. re rede deririvation vati on or restock restocking. ing.   An   agent agent reported reported as  prese  present nt need need not be monit monitor ored ed at subse subsequ quen entt scree scr eens ns but but it mus ustt be decla declare red d in subs subseequent reports. reports.

Sampling Sampli ng frequency frequency Every three three months. months.

4. 4.5 5 Bact Bacter eria iall an and d fung fungal al inf infec ecti tion onss   to   be moni mo nito tore red d in guine guineap apig igss

Sample Sam ple size size

Ten animals animals random randomly ly sampled sampled from each each  Bordetella bronchiseptica  bree  breedin ding g unit. unit. Dermatophytes  Pasteurella   spp. Table Table 4c Samp Sample le siz size e and agesages-gui guinea neapi pigs gs Salmonellae Streptobacill Strept obacillus us moniliform moniliformis is  Age No. No. of anim animals als Streptococci-~ Streptoc occi-~-haem -haemolytic olytic [except [except D group) [designation [design ationof of LancefieldGroup, LancefieldGroup, if possible) Weanlings Streptococcus Strept ococcus pneumoniae pneumoniae weeks (you (young ng adul adults) ts) 10-14   weeks Yersinia Yersi nia pseudo pseudotuberc tuberculosis ulosis months s (retired (retired breeders) breeders) > 6 month Tyzzer's Tyzz er's disease (clinical disease/pathodisease/pathological lesions/ serology*) *Results *Resu lts of serology serology are curren currently tly controcontroversial.

4.5.1 Additi 4.5.1 Additiona onall consid considera eratio tions ns   Examples of additi additiona onall micro microorg organis anisms ms to be monitored -when -whe n as asso socia ciated ted w with ith lesion lesions, s, - when when as asso socia ciated ted with with cli clinic nical al sig signs ns o of  f  disease, - when when the there re is ev evide idenc nce e of p per ertur turba batio tion n of physiolog physiological ical parameters parameters or breeding breeding  perf  pe rform orman ance ce,, -whe -w hen n using using spo sponta ntane neou ously sly immu immuno no-deficientt animals. deficien animals.

Candida albicans Candida albicans Chlamydia   spp. Clostridium   spp.

4. 4.6 6 Bact Bacter eria iall an and d fu fung ngal al in infec fecti tion onss   to   be moni mo nito tore red d in rab rabbi bits ts  Bordetella bronchiseptica  Pasteurella   spp. Salmonellae Streptococci-~ Strepto cocci-~-haem -haemolytic olytic (except (except D group) group) (design (de signatio ation n of Lancef Lancefield ield Group, Group, if   possi  po ssible ble)) Tyzzer's Tyzz er's disease (clinical disease/pathodisease/pathological logi cal lesions lesions / serolog serology*) y*) *Results *Resu lts of serology serology are curren currently tly controcontroversial.

4.6.1 Additi 4.6.1 Additiona onall consid considera eratio tions ns   Examples of addition additional al micro microorg organi anism smss to be monitored -whe -w hen n assoc associat iated ed with with lesion lesions, s, - wh when en associa associatedw tedwith ith clinicalsignsof clinicalsignsofdise disease ase,,

 

monitor itoring ing FElASA   health mon

rec recomm ommenda endation tions s

-when -whe n there there is evide evidenc nce e of pertu perturb rbati ation on of   physi  ph ysiolo ologic gical al para param meters eters or bree breedin ding g .  perfo  pe rform rman ance ce,, -when -w hen using using sp sponta ontaneo neously usly immu immunod nodefieficient animals. animals.

 

9

Microsco Micro scopic pic exami examinatio nation n of fresh fresh wet wet mounts ounts of caec caecal al conten contents ts an and d of the the inner  inner  lining linin g of the the ileum. ileum. Faecal flotation.

5. 5.1. 1.2 2 Meth Method odss used used on requ reques estt in rats rats and  Clostridium   spp. Dermatophytes  Escherichia coli   (design (designatio ation n of ser serotyp otype, e, if   possi  po ssible ble))  Klebsiella pneumoniae/oxytoca  Proteus   spp.  Pseudomonas aemginosa Staphyl Sta phyloco ococcus ccus aureus aureus Trepon Tre ponema ema cunicul cuniculii -   serology Yersinia Yersin ia pseudotubercu pseudotuberculosis losis An agen agentt must be decla declare red d as prese present nt if  it is identif identified ied in one one or m mor ore e of th the e animals anim als screene screened. d. The results results must must concontinue tin ue to be repo reporte rted d as posit positive ive at subseq sub sequen uentt screens screens until until the agent has  been  be en erad eradica icated ted by mea eans ns of e.g. e.g. re rede deririvation or restock restocking. ing. An agent agent reported reported as  pres  presen entt need need not not be monito onitore red d at sub subse se-quen qu entt scree screens ns but but it must ust be decla declare red d in subsequent subseq uent reports.

Sampling Sampli ng frequency frequency Every six months.

Sample Sam ple size size A minim inimum 8 anim animals als ra rand ndom omly ly sam sample pled d from each breeding unit.

organismss below below,, though though mice mice on only ly   The organism only onl y rare rarely ly isolated isolated from from laborato laboratory ry rats an and d mice ice,, may pres presen entt a haza hazard rd to investigators investiga tors and experimental experimental investiga inve stigation tions. s. Tests Tests for these these organi organism smss may ma y be perform performed ed on reques request. t. Se Serolo rology gy for   Encepha Encephalit litozo ozoon on cunicul cuniculii Se Serolo rology gy for   Toxop Toxoplas lasma ma gondii gondii Urine Ur ine sedime sedimentat ntation ion for  Trichosomoides   Trichosomoides crassicauda Histop His topatho athology logy of kidneys kidneys for  Klossiella  Klossiella   spp.

5. 5.2 2 Re Repor porti ting ng resu result ltss The followin The following g organi organism smss must must be men entio tione ned d in the final final re repo port rt of re resu sults, lts, with wi th a dec declar larati ation on of whe whethe therr the orga organis nism m has has been been dete detecte cted d or not not (n (num umbe berr of  anima ani mals ls positive positive), ), or not not tested tested (NT): (NT): All arthropods (identifi (ide ntificati cation on as far as po possib ssible le to the specific name) All helminths (identifi (ide ntificati cation on as far as po possib ssible le to the specific name)  Eimeria   spp. (identific (ide ntificatio ation n as far as possible possible to the specific name) Giardia   spp.

guinea gui nea pigs, pigs,   rabbits)

  spp. Spironucleus Other flagellates flagellate s (identification (identifica tion of species unnecessar unnecessary) y)  Klossiella   spp. (mous (m ouse, e, rat and guineap guineapig ig only) only)  Encephalitozoon cuniculi (comp (co mpulso ulsory ry in rabbit rabbit and guineap guineapig) ig) Toxopla Tox oplasm sma a gondii gondii (comp (co mpulso ulsory ry in rabbit rabbit and guineapig) guineapig) Trichosomoi Tricho somoides des crassicauda crassicauda (rat only)

5.1 Method Methodolo ology gy

5. 5.3 3 Mous Mouse, e, rat, rat, hams hamste terr

Table Tabl e 4d

Sample Sample si size ze and agesages-rabb rabbits its

 Age

No. of anim animals als

12-14  weeks (young adults)

~4

breeders)) >  6 months (retired breeders

~4

5.   Parasit Parasitolo ology gy (mice, (mice, rats, rats, hamste hamsters, rs,

5. 1.1   Rou tine tine meth method odol olog ogyy

at  necropsy   Examin Examinatio ation n of the pelt pelt (ski (skin n an and d hair) hair) with with the use use of d diss issec ectin ting g micr icrooscope.

 pig breeding units Sampling frequency Sampling frequency Every Ever y three months. months.

an and d guin guinea ea--

 

10

 

FELASA FEL ASA hea health lth

monito monitorin ring g

Sample Sam ple size

7.   Appe Append ndix ix I

Ten anim Ten animals rando randoml mly y sample sampled d fro from m each each  bree  breedi ding ng unit. unit.

 Necr  Ne crops opsy y proced procedur ure e

Ta Tabl ble e Sa hamste ham sterr

Sam Sample size size and age agess-mo mous use. e.

rat. rat.

and guin guineap eapig ig No. of ani animal mals s

 Age Weanlings weeks s (yo (young ung adults adults)) 10-14   week

;;;'4

>   6 mon months ths (re (retir tired ed bre breede eders) rs)

;;;'4

recomm recommend endati ations ons

This ap This appen pendix dix is a sugge suggested sted guid guidelin eline e for a necropsy necr opsy procedur procedure e for a microbio microbiologica logicall ex exam amina ination tion.. The The proce procedur dure e must be detaile detailed d in an approp appropriat riate e SOP. SOP. It may be neces necessar sary y to vary the techni techniqu ques es an and d any varian variances ces must be recorde recorded d in ad additio ditional nal SOPs. 7. 7.1 1 The The an anim imal al shou should ld be give given n a uniqu unique e necro necropsy psy number number which which can be used used for the identif ide ntifica ication tion of all sample samples. s.

5.4 Rabbi Rabbitt breed breedin ing  g   unit

7.2 Any Any clinica clinicall signs signs should should be record recorded. ed.

Sampling Samplin g frequency frequency

7.3 The The specie species, s, breed, breed, strain, strain, specia speciall feafeatures, ture s, sex, sex, age an and d weigh weightt of the the an anim imal al are recorded.

Every six months.

Sample Sam ple size Eight an Eight anim imals als rando randoml mly y sample sampled d from from each each  bree  breedi ding ng unit. unit.

7. 7.4 4 The The an anim imal al is eutha euthana nase sed d an and d th the e method me thod is recorded. recorded.

Ta Tabl ble e Sb

7.5 The The an anim imal al may may be blood blood-sa -sam mple pled d at this stage stage if small. small.

Samp Sample le si size ze an and d ag ages es-r -rab abbi bits ts No. of ani animal mals s

 Age 12-14   weeks weeks (yo (young ung adults adults))

>6

months mont hs (re (retire tired d

bre breede eders) rs)

6. Pat Patho holog logy y The following organs shou should ld be monitore monitored d for abn abnorm ormaliti alities es at routine routine necrop necropsy: sy: skin, skin, orall ca ora cavity vity,, saliva salivary ry glands glands [rat [rat onlyL onlyL respira respira-tory system, system, aorta aorta (rabbi (rabbitt onl onlyL yL heart, heart, liver, live r, spleen spleen,, gastro gastro-int -intest estina inall tra tract, ct, kidne kid neys, ys, ad adren renals, als, urogen urogenital ital tract tract (includ (inc luding ing testesL testesL body body lymph lymph nodes nodes.. A suggested suggested necropsy necropsy procedur procedure e is outline out lined d in Appendi Appendix x I. The Th e resu results lts of the the necr necrop opsy sy shou should ld be  pres  presen ented ted in th the e heal health th monito onitorin ring g re repo port. rt. All spontaneo spontaneously usly diseased diseased or suspecte suspected d diseased disea sed animals should should be considere considered d valua valuable ble source sourcess of infor inform mation ation and sho should uld  be ex exam amin ined ed at necr necrop opsy sy fo forr an any y ab abno norm rmaalitiess or lesion litie lesions. s. Al Altere tered d organ organss sho should uld be invest inv estiga igated ted by histop histopath atholo ology gy or other other test method ethodss ap appro propria priate te for eluci elucida dating ting the ae aetiolo tiology gy of the lesion[ lesion[s) s) found found..

7.6 Exter External nal exam examination ination,, includ including ing orifice orif icess and eyes, eyes, is made. made. Any Any identif ide ntifica ication tion marks marks [e.g. [e.g. tattoos tattoos)) an and d lesionss are recorded. lesion recorded. 7.7 Exam Examina ination tion is made made for ectopar ectoparasit asites es (see Section Section 5). 7.8 Samples Samples are collected collected for mycolo mycological gical examination. 7. 7.9 9 The The an anim imal al is pinne pinned d on aboar aboard, d, ventra ventrall surfac surface e upperm uppermost ost.. The The surfac surface e of  the body body may may be gently gently swabb swabbed ed to dam dampen pen th the e hair hair bu butt ca care re mus mustt be take taken n to  prev  preven entt the the fluid fluid en enter terin ing g an any y orifi orifice cess or  lesion les ionss to be sample sampled. d. 7. 7.1 10 The The skin skin is cut fr from om the mid-lin id-line e (v (ven entra trally lly)) of the the jaw jaw to th the e vulv vulva a or  scrotu scr otum. m. The The skin skin is reflec reflected ted dorsall dorsally y and  pinn  pinned ed to the boar board. d. 7.11 7.1 1 The The subcu subcutan taneo eous us tissues, tissues, includ including ing main bod body y lymph lymph nodes nodes,, mam mamma mary ry gland glandss an and d sali salivar vary y glands glands are examine examined. d. The The nutritio nutritional nal state state should should be evalua evaluated ted..

 

FELASA FEL ASA health health

monit monitori oring ng

rec recom ommen mendat dation ions s

7.12 The 7.12 The nasal nasal and oral oral cav cavities ities are opene opened d and examine examined. d. Sa Samp mples les may now be colcollected lec ted from the upper upper respirator respiratory y tract tract for   bacte  ba cterio riolog logy y an and d mycop ycoplas lasm mology ology..

11

users of labor users laborator atory y anima animals ls who who are re repo porti rting ng on the healt health h monito onitorin ring g of their  their  an anim imal al co colon lonies ies may us use e the words words 'in accordance with wit h FELA FELASA SArecomm recommendations' endations' wher wh ere e that that is in fact fact the ca case se..

7.13 7.1 3 The The thorax thorax is opened opened by two paralled paralled incision inc isionss along along the costo-ch costo-chond ondral ral jun junctio ction. n. 7.14 The 7.14 The animal animal may may be blood blood samp sampled led from fro m the heart heart at this this sta stage ge if large large.. 7.15 The 7.15 The thoracic thoracic organs organs,, beg beginni inning ng wit with h the lary larynx, nx, are exami examined ned  in si situ tu   and then remove rem oved. d. The The lungs lungs are sam sample pled d for   bacte  ba cterio riolog logy. y. The The lungs lungs,, hear heartt an and d thy thym mus are opened and examined. examined. 7.16 7.16 The The ab abdo dom minal inal wall wall is cut by an incision inc ision along along the  linea linea alba alba   and on either  side sid e of the co costa stall arc. arc. 7.17 7.1 7 The The abdom abdomina inall cavity cavity is examine examined d  before  befo re re rem moval oval of the gastr gastroin ointes testin tinal al tra tract, ct, liver, live r, spleen, spleen, kidney kidneyss and u urog rogenit enital al tract. tract. The Th e nutritio nutritional nal state state should should be evaluate evaluated. d. 7.18 The liver 7.18 liver is inspected inspected and samples samples collect col lected ed for bacte bacteriolo riology gy from from cut surface surfaces, s, if necessary. 7.19 The gastro-in 7.19 gastro-intest testinal inal tract tract is opened opened and examine examined. d. Sa Samp mples les are collecte collected d for   bacte  ba cterio riolog logy y an and d para parasit sitolo ology gy (s (sec ectio tions ns 4 and 5). 7.20 7.2 0 The genital genital organs organs and and the urinary urinary  bladd  bla dder er are are ex exam amine ined. d. 7.21 The kidneys 7.21 kidneys and adrena adrenall gla glands nds are exami exa mined ned.. The kidneys kidneys are bis bisecte ected d longitudinally. 7.22 7.22 The The ao aorta rta,, includ including ing the tho thora racic cic an and d abdom abd ominal inal portion portions, s, is opened opened and examined exam ined (rabbit (rabbit only).

8. Ap Appe pend ndix ix II Guidel Guid elin ines es for for the the us usee of th thee FELA FELASA SA  Approved Health Monitoring Report  While FELASA FELASAcann cannot ot accept accept respo responsibility nsibility fo forr tests tests or their impli implica catio tions ns,, bree breede ders rs or 

8. 8.1 1 Gene Genera rall in info form rmat atio ion n on eac each h repo report  rt  The Th e title title of the report report shou should ld be FELASA Approved Health Monitoring Report. This Th is word wording ing ca can n only only be used used if the metho me thods, ds, frequen frequency, cy, sample sample size and specie spe cies-lis s-listt of organis organisms ms monito monitored red are in full accor accordanc dance e with the recommen recommendations dations  publi  pu blish shed ed by FELA FELASA SA.. At the top of each each re repo port rt shou should ld be: be: date date of the the report, report, date animals animals screen screened, ed, identific iden tificatio ation n of all strain strains/sto s/stocks cks within within the unit, number number of anim animals als screen screened, ed,  bree  breede der's r's co code de for the unit, unit, date date when when the colony colo ny was was establish established ed and month month an and d year wh when en it was was last last re rede deriv rived ed or  restocked. Descriptio Descri ption n of the strain/stoc strain/stock k screen screened ed as follows: follows: name name of the specie species, s, followe followed d (in parenthe parentheses ses)) by the ICLA ICLAS S designa designation tion..

8.2 8.2 Layay-out out of the the repor reportt with with resp respec ectt to mi micr croo oorg rgan anis isms ms moni monito tore red d an and d the the colony col ony status status Except for gener Except general al inform informatio ation n (see (see section section repo port rt is divide divided d into three three 8.1)   the re co colum lumns ns,, the first first lis listin ting g the microorga micr oorganisms nisms monitored monitored [section 8.2.1), 8.2.1), the seco second nd recor recordin ding g the histor historica icall status status of  theing colo colony (sec (section tionof8.2.2) 8.2 .2)la and third thir giv giving the thny e re resu sults lts the lates testt the scre screen en d (section   8.2.31,   and the labora laboratory tory carryin carrying g outt the test an ou and d the met etho hod d us used ed..

8.2.1 Listin 8.2.1 Listing g of microo microorga rganis nisms, ms, method methodss and name namess of monito monitorin ring g labora laborator tories ies The organi organism smss detailed detailed in these these recom recom-menda me ndation tionss should should be listed alphab alphabetic etically ally in the their ir ap appr prop opria riate te secti section ons, s, in the orde order: r: viruses, bacteria, mycoplasm mycoplasma, a, fungi, endoparasites, endop arasites, ectoparasites. ectoparasites. Ifan abbrev abbreviatio iationis nis used used fora micro microorg organis anism, m, it must be that that used used in these these re reco com mmen enda datio tions ns.. The Th e method method used used in serolog serological ical testing must ust be g give iven n next next to the nam name of the the organism. organ ism. Abbreviation Abbreviationss used for methods methods

 

12

 

must be in accor accorda danc nce e wi with th tho those se listed listed in Appendix Appe ndix ill of these recommen recommendations. dations. The abbrev abbreviate iated d name name of the labora laboratory tory ca carr rryin ying g out out the test test must ust be reco record rded ed for  each organism/ag organism/agent. ent. Wher here e both a method method and labo laborato ratory ry nam name are are to be reco record rded ed,, they they sho should uld be in the order: order: microorganism microorganism,, laboratory, laboratory, method. Wher here e an abbrev abbreviatio iation n is used used for the nam name of the the labor laborato atory ry,, the fu full ll nam name must ust  be given given at the botto bottom m of the re repo port. rt.

8. 8.2. 2.2 2 Hist Histor oric ical al stat status us of the the colon colonyy Agains Ag ainstt each each organi organism sm must must be recorded: Poss Po

if the the orga organi nism sm has ever ever bee been n detec detected ted in an anim imals als from from the co colon lony y (i.e. positive).

 Neg  Ne g if the organ organism ism has has neve neverr been been detec detected ted in an any y scre screen en of the the an anim imals als from the colony colony [i.e. [i.e. negativ negative). e).  NT if the organ organism ism has has neve neverr been been include inc luded d in the health health monitor monitoring ing  progr  pro gram amm me (i. (i.e. e. not not tested tested). ).

FELASA FEL ASA hea health lth

o

 NT

 

monito monitorin ring g

rec recomm ommend endati ations ons

if the the org organism has no not bee been dete detect cted ed in the the curr curren entt scre screen en of  animals anim als from the colony colony.. if the orga organis nism m has has not not been been incl includ uded ed in the the curr curren entt scre screen en of  animals anim als from the colony colony..

The results The results of patholo pathologica gicall exami examinati nations ons should sho uld be recorded recorded as follows: follows:

 Pathological macroscopic lesions were we re/w /wer eree no nott obser observed ved in th thee orga organs ns examined. Pa Patholo thologica gicall change changess should should be listed sepa separa ratel tely y fo forr ea each ch strain strain withi within n the  bree  breedin ding g unit. unit.

8.3 Additio Additional nal

inf inform ormati ation on

Any additiona Any additionall inform informatio ation n should should be given given on a sep separa arate te sheet sheet accom accompan panyin ying g the main main re repo port rt an and d not on the   FELASA-Approved    itself.  Health Monitoring Report  If infecti infection on is discove discovered red betw between een tests, tests, users use rs should should be inform informed ed imme immediat diately. ely.

9. Appe Append ndix ix III

 A bb re vi at io ns 8. 8.2. 2.3 3 Curr Current ent healt health h moni monito tori ring ng re resu sult ltss of     Abb the colony colony   Ea Each ch organis organism m must must be CAR CA R bacillus bacillus Cilia-as Cilia-associated sociated respiratory respiratory recorded:  bacillus  bacil lus ELISA Enzyme-linked Enzyme -linked immuno No.. Pos  No Pos if the orga organis nism m has has been been detec detected ted sorbentt assay sorben in the cu curre rrent nt scr scree een n of anim animals als HI Haemag Hae magglutination glutination inhibition from the colony colony (numb (number er of  assay

animals anim als positive).

IF IFA A

Immunofluo Imm unofluoresce rescence nce assay

 Note: Repri  Note: Reprints nts of this this Re Repo port rt are are av avail ailab able le fr free ee of ch char arge ge fr from om the Secr Secreta etary ry,, FELA FELASA SA,, Rijksun Rijk sunive iversite rsiteit it Utrech Utrecht, t, Burea Bureau u Pro Proefd efdierd ierdesk eskund undige ige,, Postbus Postbus 80.16 80.166, 6, 3508 3508 TD Utrech Utrecht, t, The Netherlands. Netherlands.

Sponsor Documents

Hide

Forgot your password?

Or register your new account on INBA.INFO

Hide

Lost your password? Please enter your email address. You will receive a link to create a new password.

Back to log-in

Close